High performance liquid chromatography has the characteristics of high separation efficiency, fast analysis speed, and wide application range, especially suitable for the separation and analysis of compounds with high boiling points, large molecules, strong polarity, and poor thermal stability.

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High performance liquid chromatography is an important branch of chromatography. This method has become an important separation and analysis technique in disciplines such as chemistry, medicine, industry, agriculture, commodity inspection, and legal inspection. Taking the pharmaceutical industry as an example: a comparison table of the items and quantities of high-performance liquid chromatography methods included in the Chinese Pharmacopoeia.